Biophysical methods to aid protein crystallization in a pharmaceutical setting

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چکیده

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Introduction to protein crystallization.

Biological macromolecules can be crystallized by a variety of techniques, and using a wide range of reagents which produce supersaturated mother liquors. These may, in turn, be applied under different physical conditions such as temperature. The fundamental approaches to devising successful crystallization conditions and the factors that influence them are summarized here. For the Novice, it is...

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Many of the biophysical techniques developed to characterize native proteins at equilibrium have now been adapted to the structural and thermodynamic characterization of transient intermediate populations during protein folding. Recent advances in these techniques, the use of novel methods of initiating refolding, and a convergence of theoretical and experimental approaches are leading to a det...

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From Form to Function: Crystallization of Active Pharmaceutical Ingredients

S ince the introduction of aspirin in 1899, and more particularly since the advent of antibiotic ‘‘wonder drugs’’ in the 1940s, society has come to rely on the widespread availability of therapeutic drugs at reasonable prices. It was a tremendous challenge to bring penicillin to market and could not have been done without the simultaneous development of both product and process under the inspir...

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A pedestrian guide to membrane protein crystallization.

Membrane protein structural biology is a frontier area of modern biomedical research. Twenty to thirty-five percent of the proteins encoded by an organism's genome are integral membrane proteins. Integral membrane proteins, such as channels, transporters, and receptors, are critical components of many fundamental biological processes. Also, many integral membrane proteins are important in biome...

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ژورنال

عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances

سال: 2019

ISSN: 2053-2733

DOI: 10.1107/s2053273319095056